Trehalose (low endotoxin)

1. Product Description

Trehalose (Trehalose) is a non-reducing disaccharide composed of two glucopyranose molecules with a 1,1-glycosidic bond, with a relative average molecular mass of 378.33. Trehalose is divided into anhydrous and dihydrate, anhydrous has strong hygroscopicity, dihydrate has low hygroscopicity. Trehalose has a magical protective effect on a variety of bioactive substances. Under the harsh environmental conditions of high temperature, high cold, high osmotic pressure and dry water loss, a unique protective film can be formed on the surface of the cell, which can effectively protect the protein molecules from denaturation and inactivation, so as to maintain the life process and biological characteristics of the living body, so it is also known as "the sugar of life".

2. Product advantages

Meet the requirements of different regulations and market standards: CP/USP/EP/JP;

Higher purity and lower impurities: strict control of organic impurities and inorganic impurities in products, strict control of inorganic impurities by controlling chloride, sulfate and ignition residue, and comprehensive control of organic impurities by controlling solvent residues, related substances, soluble starch, solution clarity and color, and enzyme residues;

Customized packaging: different sizes of packaging can be used according to customer needs;

Higher safety: low endotoxin sugar strictly control microorganisms and endotoxin, using ultrafiltration, recrystallization and other processes to effectively remove bacterial endotoxin.

3. Product Application

freeze-drying protective agentTrehalose has good stability and safety, low hygroscopicity, and has a higher glass transition temperature than sucrose. Therefore, in biological products, trehalose is mainly used as a protective agent, which has a good freeze-drying protection effect on proteins. It is recognized as one of the most widely used and most effective freeze-drying protectants.

enzyme stabilizerThe product can be used as an enzyme protective agent for DNA polymerase, reverse transcriptase and other tool enzymes stabilizer, so as to PCR, RT-PCR, qPCR and other experimental reaction optimization of the additive components, greatly improve the amplification efficiency and amplification specificity, improve reverse transcription efficiency.

1) Trehalose has thermal stability and thermal activation characteristics. Trehalose can maintain the protein skeleton and its natural structure in the process of protein thermal denaturation. It has been validated in a variety of enzymatic reactions in molecular biology, such as cDNA synthesis with the participation of reverse transcriptase, DNA degradation with the participation of Dnase I, and restriction enzyme cleavage reactions;

2) Trehalose can be used as a PCR enhancer. The stability of Taq DNA polymerase was maintained by reducing the melting temperature of DNA double strand, and the efficiency of PCR was improved;

3) Trehalose can neutralize the inhibitory effect of inhibitors on DNA polymerase and maintain the efficiency of PCR amplification. Trehalose, as a real-time fluorescent quantitative PCR additive by SYBR Green I method, effectively reduced the inhibition of SYBR Green I on PCR;

4) trehalose can be used as a rare sample genome or very low concentration of genome preservation agent.

4. Characteristic R & D service

1. National Pharmacopoeia Detection Operation Decomposition Guidance

2. Customized specialty preparation services