Poloxamer 188

1. Product Description

Poloxamer 188 is a block copolymer composed of a polyoxyethylene segment and a polyoxypropylene segment, the block copolymer being terminated by two hydroxyl groups. At room temperature for white to yellowish translucent waxy solid. 18 in the 188 name of the poloxamer means that the molecular weight of the polyoxypropylene segment is about 1800, and the latter digit 8 means that the mass ratio of the polyoxyethylene segment is about 80%. The average molecular weight of the polymer was 7680-9510. Structurally, the polyoxypropylene segments in the polymer are hydrophobic, while the polyoxyethylene segments are hydrophilic, which makes the poloxamer 188 both lipophilic and hydrophilic. The poloxamer 188 is thus a non-ionic surfactant.

2. Product advantages

Higher purity: Poloxamer 188 using the company's unique purification process;

Better appearance: almost white appearance, resulting in better quality;

Higher safety: strict control of heavy metals and inorganic salt residues in products;

Good emulsifying effect: to ensure the stability of the preparation during the replacement of excipients;

Lower impurity residue: strict control of ethylene oxide, propylene oxide, dioxane impurity residue;

Good packing: the inner packing material is polypropylene plastic bottle.

3. Product Application

surface active agentPoloxamers are a class of amphiphilic, nonionic surfactants. In particular, high-purity poloxamer 188, which are widely used in biopharmaceutical preparations, are self-assembled into micelles through poloxamer 188 in water, making them ideal drug transport carriers. For example, ornidazole injection and propofol injection utilize the emulsification of poloxamer 188 to promote the dispersion of the drug in the human body. The addition of poloxamer 188 to diazepam injection significantly reduces the precipitation of crystals after exposure of the injection to blood, which can cause thrombophlebitis. In addition, poloxamer 188 can be used as a solid dispersant and increase the dissolution rate of the drug concerned to promote the absorption of the drug, for example, for selepin, digoxin, phenylbutazon, etc. On the basis of solubilizing drugs, poloxamer 188 as a matrix can play a slow-release and prolonged effect, such as for griseofulvin latex cream, compound metronidazole suppository, indomethacin suppository and so on.

In addition, poloxamer 188 has a wide range of applications in the separation and purification of protein drugs, mainly because of the following advantages:

1) Good biocompatibility;

2) low toxicity;

3) Below room temperature, various proteins in plasma have different solubilities in poloxamer 188 solution and can form insoluble complexes, and poloxamer 188 do not hemolyze at low temperature. At present, it has been reported that this method has been applied to the separation and purification of albumin, immune serum globulin, concentrated preparation of factor 8 and complex prothrombin.

vaccine adjuvantVaccine adjuvants are substances that can non-specifically change or enhance the body's specific immune response to antigens and play an auxiliary role. TLR4 receptor agonists are a class of emulsions of glucopyranoside lipid adjuvants comprising squalene, glycerol, phosphatidylcholine, glucopyranoside lipid (GLA), poloxamer 188, ammonium phosphate buffer. Studies have shown that the emulsion adjuvant can enhance the immune response of CD4 T cells to malaria antigens.

repair cell membraneThe shear force generated by impeller agitation at a certain stirring rate generally does not destroy the cells. The shear causes the bubbles to move upward and collapse at the gas-liquid interface. However, the collapse of small bubbles will release a large amount of energy, which will destroy the stability of the cell structure in the bubble rich liquid layer. In addition, studies have shown that high rates of gas entering the liquid can also cause cell death. Poloxamer 188 has amphiphilic in molecular chain structure, can significantly reduce the surface tension between cells and bubbles, thus reducing the influence of surrounding shear stress. It has been shown that shear protection additives such as poloxamers 188 reduce physical damage to animal cells in suspension by physical and biochemical measures. Poloxamer 188 can be directly embedded in the lipid layer of cell membrane to protect cells, and has been widely used in the study of cell membrane repair therapy. For example, in the related patent reports on the transplantation of fat plant cells, the addition of poloxamer 188 is beneficial to the sealing and stabilization of cell membrane, can effectively repair the damaged fat cell membrane, and improve the viability and survival stability of the damaged fat cells. In patent reports related to mammalian cell culture, the percentage of viable cells in the medium is high above a certain concentration of poloxamer 188.

4. Characteristic R & D service

1. National Pharmacopoeia Detection Operation Decomposition Guidance

2. Customized specialty preparation services